Chinese university students showed less disordered eating during the COVID-19 campus lockdown.

OBJECTIVE: The rapid spread of the Omicron variant of COVID-19 in China had resulted in campus lockdown in many universities since February 2022, profoundly affecting students' daily lives. Campus lockdown conditions differ considerably from home quarantine, so that the eating patterns of university students may be different. Thus, the current study aimed to: (1) investigate university students' eating patterns during campus lockdown; (2) identify factors associated with their disordered eating. METHOD: An online survey about recent life changes, disordered eating, stress, depression, and anxiety was carried out from April 8th to May 16th, 2022. A total of 2541 responses from 29 provinces/cities of China were received. RESULTS: 2213 participants were included in the main analysis, and other 86 participants were analyzed separately as a subgroup due to their diagnosis of eating disorder. Participants who were undergoing campus lockdown (the lockdown group) showed less disordered eating than those who had never been in campus lockdown (the never-lockdown group), as well as those who had experienced campus lockdown before (the once-lockdown group). However, they perceived more stress and felt more depressed. Being female, higher BMI, gaining weight, increasing exercise, spending more time on social media, higher level of depression and anxiety were all related to disordered eating in the lockdown group. CONCLUSIONS: Disordered eating among Chinese university students was less prevalent during campus lockdown due to the strict and regular diet. However, there is a potential risk of "revenge eating" after campus lockdown ends. Thus, there should be further tracking and related prevention. LEVEL OF EVIDENCE: IV, uncontrolled trials without any interventions.

Determination of the minimum detection limit of the new coronavirus 2019-nCoV nucleic acid detection kit (fluorescence PCR method)

Objective To determine the minimum detection limit of the novel coronavirus 2019-nCoV nucleic acid detection kit (fluorescent PCR method) developed and produced by Shanghai Jienuo Biotechnology Co., Ltd., and to verify whether it can effectively detect the novel coronavirus at the minimum detection limit level. Methods 20 different clinical positive samples were diluted to 5 concentration gradients of 62 500, 12 500, 2 500, 500, and 100 copies/mL, and the new coronavirus 2019-nCoV nucleic acid detection kit (fluorescent PCR method) was used for 3 repetitions Test, initially determine the minimum detection limit range;use 3 batches of kits to perform 20 repeated tests on 20 different clinical positive samples diluted to 5 concentration gradients of 1,500, 700, 500, 300, and 150 copies/mL, and further Determine the minimum detection limit;another 25 different clinical positive samples were diluted to the minimum detection limit level, and 20 repeated tests were performed with 3 consecutive batches of kits to verify the minimum detection limit. Results The positive detection rate of samples was 100% at the 4 concentration levels of 62 500, 12 500, 2 500, and 500 copies/mL. At the concentration level of 100 copies/mL, the positive detection rate of samples was =55%. The minimum detection limit is between 500 and 100 copies/mL;after 20 repeated determinations, the positive detection rate of the sample at the 3 concentration levels of 1, 500, 700, and 500 copies/mL is =95%, and the positive detection rate of the sample is =95%, and the positive detection rate of 300 and 150 copies/mL 2 The positive detection rate of the samples at each concentration level is less than 95%, and the minimum detection limit is determined to be 500 copies/mL;25 positive samples diluted to the minimum detection limit concentration level, the positive detection rate is more than 95%. Conclusion The minimum detection limit of the new coronavirus 2019-nCoV nucleic acid detection kit (fluorescent PCR method) developed and produced by Shanghai Genuo Biotechnology Co., Ltd. is determined to be 500 copies/mL. The sample can effectively detect viral nucleic acid and meets my country's relevant requirements for the minimum detection limit of detection reagents.

Mental health status of students' parents during COVID-19 pandemic and its influence factors.

BACKGROUND: During the outbreak of COVID-19, the national policy of home quarantine may affect the mental health of parents. However, few studies have investigated the mental health of parents during the COVID-19 pandemic. AIMS: To investigate the depression, anxiety and stress of the students' parents during the COVID-19 pandemic, and to explore the influence factors, especially the influence of social support and family-related factors. METHODS: The Generalised Anxiety Disorder-7, Patient Health Questionnaire-9, Perceived Stress Scale-10 and Social Support Rating Scale were applied to 1163 parents to measure the parents' depression, anxiety, stress and social support. RESULTS: (1) The detection rates of depression and anxiety in parents were 6.1% and 4.0%. The depression, anxiety and perceived stress of parents in central China were significantly higher than those in non-central China. The anxiety of college students' parents was lower than that of parents of the primary, middle and high school students. The depression, anxiety and perceived stress of parents with conflicts in the family were significantly higher than those with a harmonious family. Other factors that influence parents' depression, anxiety and perceived stress include marital satisfaction, social support, parents' history of mental illness and parenting style, etc. (2) The regression analysis results showed that perceived stress, social support, marital satisfaction, family conflicts, child's learning stage as well as parents' history of mental illness had significant effects on parents' anxiety and depression. CONCLUSION: During the COVID-19 pandemic, the mental health of parents was affected by a variety of factors. Good marital relationships, good social support, family harmony and parents without a history of mental illness may be protective factors for parents' mental health, while perceived stress and child in middle or high school are risk factors for parents' mental health.